111 The mechanism of YKL-40 in enhancing the vascular permeability induced by histamine

This study aims to explore the role of YKL-40 on permeability vessels in mice model and HDMECs histamine induces vascular hyperpermeability. The expression a HMC-1 was detected by RT-PCR western blot. experiment divided into different concentrations (10, 100 500ng/ml) alone,different concentrations(10, 500 ng/ml) YKL-40+100 μM histamine,500ng/ml YKL-40+100μM histamine+10 loratadine,100 alone blank control group PBS.The effects permeability,sVE-cadherin release,sVE-cadherin disruption were investigated transwell,ELISA or immunofluorescence. phosphorylation VE-cadherin,p38,and Akt,in each intervention factor,treated Western Blot.A total 30 balb/c randomly five groups:control group,histamine (1mM) treated group,YKL-40 (500ng/ml) group, histamine+YKL-40 loratadine+histamine+YKL-40 group,with six group.All injected tail vein with 1% Evan's blue solution,and then intracutaneously ear.Loratadine intraperitoneally 1h before treatment. level extravasated extracted formamide quantified spectrophotometrically at wavelength 610 nm.The mRNA VE-cadherin RT-PCR. release ELISA.The protein VE-cadherin,p38 Akt Blot.The results as follows. significantly promoted changes led released,disruption induced histamine.Furthermore,YKL-40 also enhanced p38 pathways.The same have been obtained animal experiments.As result,we suggest that may serve pro-permeability cytokines,and play pathogenesis CSU. will help further elucidate CSU provide new target for development anti-histamine resistance drugs